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Real-time PCR analysis of MLC-1a expression levels in hearts of tg/tg and ce/ce mutant Mitf mice and their wild-type littermates. mRNA was extracted from hearts of wild-type (wt) and Mitf-mutated mice. mRNA quantitation was determined by SYBR green incorporation. Expression levels were normalized to that of the β-actin housekeeping gene. Results represent the mean ± standard error (n = 14 for tg/tg and n = 13 for ce/ce mice).
