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Knockdown of alpha-4 enhances caspase 3/7 activation in response to TNF-α. HeLa cells were transfected with control siRNA or alpha-4 siRNA and treated with 40 ng/ml TNF-α, 40 ng/ml anisomycin, or vehicle for 48 h, and the cells were stained for activated caspase 3/7 using Promega Caspase-Glo 3/7. The open bars represent control siRNA, and the gray bars represent alpha-4 siRNA; *, P < 0.01, and **, P < 0.005 by Student's t test (n = 3). The error bars represent standard deviations.
