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. 2007 Apr 16;27(12):4217–4227. doi: 10.1128/MCB.00067-07

FIG. 8.

FIG. 8.

Dominant-negative alpha-4 enhances caspase 3/7 activation in response to TNF-α and suppresses growth in soft agar. (A) HEK293 cells expressing FLAG-alpha-4 DND (DN 5; gray bar) or empty vector (C 11; black bar) were treated with 40 ng/ml TNF-α or vehicle and assayed for activation of caspase 3/7 (*, P < 0.01 by Student's t test; n = 3). The error bars represent standard deviation. (B) HEK293 cells expressing FLAG-alpha-4, FLAG-alpha-4 DND, or empty vector were grown in soft agar, stained, and photographed under phase illumination (the bar indicates a length of 70 μm). (C) MDA-MB-231-luc cells expressing FLAG-alpha-4 DND (DN 2) or empty vector (C 13) were growth in soft agar and stained as previously described.