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Generation of cPGES/p23−/− mice. (A) Southern blot analyses of DNA from E18.5 embryos generated by the intercross of mice heterozygous for the cPGES/p23 mutant allele. (B) Northern blot analyses of cPGES/p23 mRNA expression in whole E18.5 embryos. Analysis with a β-actin-specific probe verified equal RNA sample loading. (C) Western blot analysis of cPGES/p23 protein expression in E18.5 liver and MEFs. β-Actin expression is shown as a loading control.
