FIG. 11.

Defective nuclear translocation of GR in cPGES/p23^−/− fibroblasts. (A) Immunofluorescent staining of transformed fibroblasts for GR after treatment with dexamethasone demonstrates defective translocation in the cPGES/p23^−/− fibroblasts. Fibroblasts were treated with dexamethasone (10 nM) for 0, 30, and 60 min in serum-free medium. Software (ImageJ, NIH) was used to determine the mean pixel density of the nucleus of representative cells in each culture. n = 12. *, P < 0.05 compared to all other groups by the Tukey-Kramer test for multiple comparisons. (B) Western blot analysis demonstrates increases in GR in the nucleus and decreases in GR in the cytoplasm after dexamethasone stimulation (10 nM) for 0, 30, and 60 min in the wild-type fibroblasts. No alterations in GR localization were observed in the cPGES/p23^−/− fibroblasts after stimulation. Sample loading was verified by expression of α-tubulin for the cytoplasmic fraction and histone H1 for the nuclear fraction. Change was determined by densitometric analysis and normalized to the loading controls.