FIG. 7.

cPGES/p23^−/− mice have alterations in expression of glucocorticoid-regulated genes in the lung. (A) RT-PCR analysis was used to determine expression levels of glucocorticoid-regulated genes, including ENaCγ and MK, in total RNA isolated from E18.5 lungs. Expression levels were normalized to β-actin, an endogenous control; the results are expressed as change (fold) relative to wild-type expression levels. cPGES/p23^−/− lungs, n = 7; wild-type lungs, n = 9. *, P < 0.05. (B) Western blot analysis of GR expression in E18.5 lungs from wild-type and cPGES/p23^−/− embryos demonstrates similar GR protein levels. β-Actin expression verifies equal sample loading.