FIG. 8.

cPGES/p23^−/− mice display abnormal liver morphology and have alterations in expression of glucocorticoid-regulated genes in the liver. (A) RT-PCR analysis was used to determine expression levels of gluconeogenic enzymes, glucose-6-phosphatase (G6pc) and serine dehydratase (Sds), in total RNA isolated from E18.5 livers. Expression levels were normalized to β-actin, an endogenous control; the results are expressed as change (fold) relative to wild-type expression levels. n = 5. *, P < 0.01. (B to E) Livers were removed from embryos at E17.5 and fixed overnight in 10% formalin. Liver sections from wild-type (B) and cPGES/p23^−/− (C) embryos were stained with hematoxylin and eosin for routine histological examination. Glycogen content in the liver was assessed by PAS staining. Note the intense staining in the wild-type liver (D) compared to the cPGES/p23^−/− liver (E). Representative results for four embryo livers analyzed for each genotype are shown. Original magnification, ×10.