FIG. 1.
Induction of Bcl-xL mRNA by GATA-1 in G1ER cells was repressed by the enforced expression of Gfi-1B. (A) G1ER cells infected with Flag-Gfi-1B-encoding (MIGR-Gfi-1B) or control (MIGR) retroviruses were treated with β-estradiol (10−7 M) to activate GATA-1, after which cells were harvested at the times indicated for RNA preparation followed by RT-PCR analysis using primers specific for mouse Gfi-1B, human Gfi-1B (for MIGR-CFlag-Gfi-1B), mouse Bcl-xL, and mouse β-actin. (B) Densitometric evaluation of relative levels of mouse endogenous Gfi-1B and Bcl-xL RNA normalized by the β-actin mRNA level in each sample of MIGR1 (left panel)- or MIGR-CFlag-Gfi-1B (right panel)-transfected G1ER cells.