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Occupancy of the Bcl-x promoter by GATA-1 and Gfi-1B in G1ER cells. In vivo cross-linked protein-DNA complexes were isolated from G1ER cells treated with β-estradiol for the indicated periods and were subjected to immunoprecipitation with IgG (control), Gfi-1B (α-Gfi-1B), or GATA-1 (α-GATA-1) antibody. The coimmunoprecipitated DNA was amplified by PCR using the Bcl-x proximal-promoter primers or TK1 primers as a negative control.
