FIG. 3.

Expression patterns of nkd1^lacZ and nkd2^lacZ. (A and B) X-Gal staining of 12.5-dpc embryos harvested from nkd1^lacZ/+ (A) or nkd2^lacZ/+ (B) heterozygous intercrosses reveals an intensity of blue staining that increases with the nkd^lacZ copy number. (C) Ten and one-half day postcoitus nkd1^lacZ/+ (left) or nkd2^lacZ/+ (right) embryo. Note dorsal CNS staining in nkd1^lacZ that is absent from nkd2^lacZ (arrows) but similar somite (arrowheads) and tail bud (asterisks) expression. (D) Fifteen and one-half day postcoitus nkd1^lacZ/+ (left) and nkd2^lacZ/+ (right) embryos. Note the similar expression of each gene in the snout, ear, and hair follicles (panels D′ to D″) but distinct staining patterns in the distal limb buds. (E and F) Liver tissue from adult nkd1^lacZ/+ (E) or nkd2^lacZ/+ (F) mice showing pericentral hepatocyte staining in the former but not the latter. (G) Snout tissue of a 15.5-dpc nkd1^lacZ/+ mouse showing expression in whisker mesenchyme (arrow) and dermal papillae (arrowhead). (H) nkd2^lacZ/+ mouse intestine tissue showing staining of the wall musculature (arrowhead).