FIG. 7.

In vivo cleavage activity of SETMAR. (A) The agarose gel shows PCR products of excision assays on plasmid DNA from HeLa cells transfected with pHsmar1-neo and the helper plasmids indicated. The faint, smeary products around the expected size in lane 2 indicate loss of Hsmar1 transposons from the donor plasmids catalyzed by the SETMAR protein. (B) The overall structures of footprints from HeLa and CHO-K1 cells. The schematic view of the donor site is depicted above. Δ represents deletions, and the sequences in the white boxes are microhomologies at the junctions.