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. 2007 Apr 16;27(12):4488–4499. doi: 10.1128/MCB.01636-06

FIG. 2.

FIG. 2.

Subcellular localization of Smad7 mutants. (A) Schematic diagram of the structure of Smad7 and its truncation mutants. Smad7(WT) represents wild-type Samd7. NLS-Smad7 contains an N-terminally fused NLS from the SV40 large T antigen. The Smad7 MH2 domain is located at the C terminus from amino acids 260 to 426. (B) The MH2 domain is important for Smad7 nuclear localization. Hep3B and HeLa cells were transfected with wild type or mutant Flag-tagged Smad7. After 40 h, their subcellular localizations were determined by indirect immunofluorescence with anti-Flag antibody and FITC-conjugated secondary antibody. Scale bars, 20 μm. (C) Nuclear localization of NLS-Smad7. Hep3B cells transfected with Smad7(WT) or NLS-Smad7 were treated with TGF-β1 for 45 min. Then, their subcellular localization was determined by indirect immunofluorescence with anti-Flag antibody and FITC-conjugated secondary antibody. Scale bar, 20 μm.