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The putative NLS of Par 6 is at least partially responsible for its nuclear localization. (A) HeLa cells were transfected with the indicated GFP constructs. (B) Quantitation of the ratio of nuclear to cytoplasmic GFP signal in HeLa cells transfected with the indicated constructs. Ratios were determined by measuring fluorescence intensity in equally sized areas within and immediately adjacent to the nucleus using Image J. Outliers are indicated by open circles.
