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Nuclear Par 6 is localized to discrete domains called nuclear speckles. (A) Top two rows, the indicated cell types were stained with Par 6 and SC-35 antibody to show colocalization. Bottom row, GFP-Par 6 MDCK cells were extracted prior to fixation and then stained with SC-35 antibody. (B) HeLa cells were stained with Par 6 antibody and either monoclonal antibody 9A9 (U1A/U2B‴) or Y12 (Smith antigen [Sm]) to show colocalization with speckle proteins.
