FIG. 8.

Par 6 knockdown results in dispersed speckles and enlarged nuclei. (A) HeLa cells were treated with the indicated siRNA constructs and stained with Par 6 and SC-35 antibodies. Dotted lines show the outline of the nucleus in the top row. (B) The nuclear fluorescence intensity for cells transfected with the different siRNA constructs was quantified from confocal images using Image J. (C) Cells were transfected with siRNA no. 2 and either GFP (rescue control) or GFP-Par 6 (rescue). Par 6 staining is shown for the control cell, while GFP signal is shown for the rescued cell. The bottom row shows staining for SC-35. (D) Cells were treated with either siControl or Par 6 siRNA no. 2. Panels show staining for U1A/U2B‴. (E) Quantitation of the nuclear area of cell subjected to the indicated treatments was done with Image J. Error bars in panels B and E represent standard deviations from the means.