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Assays for Par 6 involvement in transcription. (A) HeLa cells were pulsed with 2 mM 5′-FU for 6 min and then fixed and processed for immunofluorescence with Par 6 and BrdU antibodies. The arrow points to nucleolar incorporation of 5′-FU. (B) Quantitation of the amount of luciferase production in the GAL4 transcriptional-transactivation assay. The results represent four independent experiments. Error bars indicate standard deviations.
