FIG. 7.

Cellular distribution of the 7a protein in SARS-CoV-infected Vero E6 cells as determined by fractionation experiments. SARS-CoV-infected Vero E6 cells harvested at different time points postinfection were fractionated into two fractions, one that contained mitochondria (fraction 2) and another that contained all other cellular compartments (fraction 1). Western blot analyses were performed to determine the amounts of 7a and cellular proteins in these two fractions. The protein marked with an asterisk represents an oligomeric form of 7a (lanes 3 to 6). Molecular mass markers are shown on the left.