FIG. 3.

Analyses of PF-RC DNA. HBV (A) and DHBV (B) core and PF DNA, extracted as for Fig. 2, or the plasmid pcDNA3 (C) were heated at the indicated temperatures and analyzed by Southern blotting. The diagrams at the bottom depict that only RC, not NC, is linearized at the indicated temperatures. SC, supercoiled plasmid. (D) HBV core or PF DNA was digested with the indicated restriction enzymes and analyzed by Southern blotting. The diagram at the bottom depicts the restriction sites on the RC DNA. E, EcoRI; R, RsrII.