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. 2007 Apr 4;81(12):6164–6174. doi: 10.1128/JVI.02721-06

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FIG. 4. — Detection of HBV PF DNA in the nucleus and nucleocapsid. (A) HBV PF DNA was isolated from the whole-cell lysate, using either Hirt extraction (lane 1) or direct phenol extraction (lane 2), or from the nuclear fraction (N, lane 3) by Hirt extraction. (B) HBV DNA was isolated from the peak fractions of nucleocapsids purified by CsCl gradient centrifugation with protease digestion for core DNA (lanes 1 to 4) or without protease digestion for PF DNA (lanes 5 to 8). HBV core DNA (lane 9) and PF DNA (lane 10) were also extracted from the purified HBV nucleocapsids (fraction 6) after being mixed with the total HepG2 cell lysate. Fr no., fraction number.