FIG. 3.

Western blot comparison of the effects of various concentrations of PD98059 and U0126 on late viral protein expression in PDA cultures 4 days post-JCV exposure. Concentrations tested include the accepted effective dosages of PD98059 (20 μM) and U0126 (10 μM) found in the literature for MEK1/2 inhibition in similar cell types. Blot assays utilized whole-cell lysates that were resolved on 4 to 12% gradient gels, transferred to a PVDF membrane, and probed with anti-Vp-1 and anti-β-actin. The anti-β-actin blot serves as the total protein loading control. Results included are representative of three independent experiments.