FIG. 1.
Confocal analyses of a Hep2-derived cell line stably expressing eGFP-lamin A/C. Cells were either mock infected (Mock) or infected with 5.0 PFU/cell of wild-type HSV-1(F) (designated F) or mutant US3(K220A), lacking pUS3 kinase activity. Cells were fixed and permeabilized at 16 h postinfection and stained with polyclonal anti-pUL34 chicken polyclonal antibody, followed by Texas Red-conjugated secondary antibody, and examined with confocal microscopy. (A) Analysis of optical sections taken through the middle of cells. (B) Optical sections taken at the bottom of cells. Regions indicated in white rectangles in panels D, E, F, J, K, and L are digitally magnified in the panel immediately below them (panels G, H, I, L, M and N, respectively). Coincident signals in the merged images (rightmost column) are indicated by a yellow color. An arrow indicates one region of the infected cell containing less lamin A/C than surrounding areas.