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Association of A56/K2 with TAP-tagged A28. Cells were infected with VACV WR, vA28TAP, and vA28TAPΔK2 as indicated and lysed as described in the legend to Fig. 1; proteins were purified by affinity chromatography, resolved by SDS-PAGE, and detected by Western blotting with antibodies to the proteins A56, K2, A28, p4b, and GAPDH, as indicated. PreTAP, proteins prior to chromatography; TAP, proteins after chromatography.
