FIG. 4.
Uridylylation of E. coli-derived VPg. Electrophoretic gel indicating uridylylation of VPg which was either obtained by peptide synthesis (lanes 1 and 2 and lanes 5 and 6) or expressed as a fusion protein in E. coli (lanes 3 and 4 and lanes 7 and 8). The reactions are performed using either a poly(A) RNA template (lanes 1 to 4) or the 2CCRE RNA hairpin template (lanes 5 to 8). Uridylylated VPg peptides are labeled. Larger-molecular-weight bands in the synthesized peptide lanes (lanes 1 and 2 and lanes 5 and 6) represent peptide heterogeneity not present in the E. coli-expressed peptides.