FIG. 6.

ITC analysis of VPg mutants. Representative ITC data illustrate the effects on 3D-binding of the three classes of VPg mutations. (A) VPg mutant R17A does not bind to 3D, displaying little or no heat released upon addition to 3D. (B) VPg mutant T15A binds to 3D with a somewhat reduced affinity, displaying smaller peaks and a shallower curve than wild-type VPg but still allowing for accurate determination of thermodynamic binding parameters. (C) VPg mutant Y3A binds to 3D much like wild-type VPg, displaying similar thermograms and allowing for accurate determination of thermodynamic binding parameters.