TABLE 1.
Estimations of the levels of viral proteins, viral DNA, and infectivity in extracellular and intracellular virion particles produced in cells infected with the pp28STOP61 or pp28STOP50 viruses
| Virus and domaina | MCPb | gB | pp28 | pp65 | gB/MCP ratioc | pp28/MCP ratioc | pp65/MCP ratioc | DNA copy numberd | Infectivity leveld |
|---|---|---|---|---|---|---|---|---|---|
| pp28STOP61 | |||||||||
| IC (6) | 4.67 | 12.75 | 7.56 | 10.27 | 2.7 (0.05) | 1.6 (0.02) | 2.2 (0.10) | 1.7 × 109 (9.22) | 6.00 |
| EC (5) | 9.74 | 27.96 | 14.40 | 20.36 | 3.1 (0.10) | 1.58 (0.08) | 2.14 (0.14) | 6.9 × 109 (9.84) | 8.47 |
| pp28STOP50 | |||||||||
| IC (7) | 3.13 | 5.00 | 1.95 | 22.10 | 1.64 (0.07) | 0.65 (0.06) | 6.92 (0.23) | 1.1 × 109 (9.04) | 4.75 |
| EC (6) | 3.56 | 10.59 | 3.74 | 7.23 | 3.06 (0.07) | 1.04 (0.01) | 1.93 (0.05) | 8.9 × 108 (8.95) | 6.91 |
The numbers in parentheses represent fractions containing peak infectivity. IC, intracellular; EC, extracellular.
The densities of the corresponding proteins in peak fractions from Fig. 6C are shown.
The ratios of envelope (gB), tegument (pp28), and tegument (pp65) proteins to MCP were calculated for the peak fractions of infectivity. Values represent the means (standard errors) of the results of three to four independent experiments. Results of a single representative experiment are shown in Fig. 6C.
The viral DNA copy numbers in parentheses and infectivity levels are represented in log scale for the peak fractions of infectivity. Note that prior to application to the gradients, the total numbers of extracellular viral particles from HF (as analyzed in clarified supernatant) that had been infected with the pp28STOP61 and pp28STOP50 viruses differed by comparable levels of infectivity (1.3 logs) and viral DNA copy number (l log).