TABLE 2.
Recovery kinetics of the pp28 and pp28Mut proteins in FRAP assays
| Rate type | Ratio of fluorescence recoverya
|
|||
|---|---|---|---|---|
| pp28WT | pp28Mut61 | pp28Mut50 | pp28Mut35 | |
| Recovery in AC | 1.6 | 2.0 | 2.2 | 1.4 |
| Recovery in cytoplasm | 1.1 | 1.2 | 2.0 | 1.5 |
| Accumulation in AC | 1.5 | 1.7 | 1.1 | 0.9 |
a
Ratios of fluorescence recovery are based on the ratio of fluorescence at 20 min to the fluorescence at 0 min. For the kinetics of accumulation in AC, i.e., localization in the AC, the ratio of fluorescence recovery in the AC to the fluorescence recovery in the cytoplasm was calculated. Note that the accumulation rate of pp28Mut35 in AC was <1.0, indicating that, at equilibrium, the majority was localized outside of the AC, whereas pp28WT had an accumulation rate of 1.5.