FIG. 6.

Western blot analyses of CMV 2b and CP in CMV-inoculated T2 generation plants. Total plant proteins were extracted from upper uninoculated leaves of two m-1-2 T2 plants (m-1-2-1 and m-1-2-5), two s-4-13 T2 plants (s-4-13-9 and s-4-13-11), and two T2 plants of the control vector-transformed plant line CK-17-27 (CK-17-27-1 and CK-17-27-4) at 15 dpi. Total protein extracted from an uninoculated tobacco plant (CMV−) was used as a negative control. These four sRNA-transgenic T2 plants showed no CMV symptoms, whereas the two control plants exhibited typical mosaic symptoms. The plant proteins were subjected to immunoblot analysis using antibodies against glutathione S-transferase-2b fusion protein (A) or CMV CP (B). Coomassie Bright Blue staining of the large subunit of ribulose 1,5-bisphosphate carboxylase/oxygenase indicates equal loading.