FIG. 9.

(A) GfV morphogenesis occurring in the ovarian calyx epithelium. Envelopes (arrows) appear to assemble de novo on the surfaces of an intranuclear virogenic stroma (VS); several nucleocapsids (NC) are packaged within each envelope. (B) Particles exit the nucleus (N) through the nuclear envelope (NE) into the cytosol (C), where the two membranes derived from the nuclear envelope are, in some as-yet-undetermined manner, lost, such that only the original envelope (IM) is retained. (C) Virus release by budding through the plasma membrane (PM) into the lumen (L) of the calyx; mature, extracellular virions thus have two envelopes: IM, inner membrane; OM, outer membrane. Bar, 200 nm.