Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Feb 21;81(9):4677–4693. doi: 10.1128/JVI.02691-06

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007, American Society for Microbiology

PMC Copyright notice

FIG. 5. — Immunophenotypic analysis of thymic and peripheral T lymphocytes from CD4C/HIV-Nef^allele Tg mice. Thymus (A) and peripheral LN (pLN) (B) cells from a representative Tg mouse with each allele (CD4C/HIV-Nef^AD93 [F115820], CD4C/HIV-Nef^032an [F95582], CD4C/HIV-Nef^039nm [F75581], CD4C/HIV-Nef^JR-CSF [F104995], CD4C/HIV-Nef^YU10x [F92751], CD4C/HIV-Nef^SF2 [F92748], CD4C/HIV-Nef^NL4-3(T71R) [F101376], and CD4C/HIV-Nef^NL4-3(WT) [F27367]) and from a non-Tg littermate were analyzed the same day by flow cytometry for the expression of CD4 and CD8. The percentage of cells found in the relevant quadrant is indicated on the left of the colon, while the underlined number on the right refers to the mean fluorescence intensity. Note the presence of two subsets of CD4⁺ T cells (CD4^high and CD4^low) in pLN cells expressing Nef^039nm and to a lesser extent in those expressing Nef^SF2 and Nef^JR-CSF. These correspond, respectively, to cells expressing low and high levels of Nef, as previously reported for Tg mice expressing Nef^NL4-3(WT) (103). These data are representative of at least three independent experiments with 4 to 10 mice. (C) Three-color FACS analysis (CD4-allophycocyanin, CD8-fluorescein isothiocyanate, and CD44-PE) was performed on pLN cells from a representative mouse from CD4C/HIV-Nef^AD-93 (F115820), CD4C/HIV-Nef^032an (F95582 and F95581 [not shown]), CD4C/HIV-Nef^039nm (F75581 and F115817 [not shown]), CD4C/HIV-Nef^YU10x (F92751), CD4C/HIV-Nef^JR-CSF (F104995), CD4C/HIV-Nef^SF2 (F92748), or CD4C/HIV-Nef^NL4-3(T71R) (F101376 and F101374 [not shown]) Tg lines and from a non-Tg littermate. Isotype control antibody was used as a negative control. Data are shown only for the CD4⁺ T-cell population. The results shown are representative of at least two or three independent experiments. (D) Table representing ratios (Tg/non-Tg) of the percentages of cells expressing (high for CD25, CD44, and CD69) or not expressing (negative/low for CD62L and CD45RB) the indicated cell surface marker, as shown in panel C. Four to six Tg mice of each allelic line along with their respective non-Tg littermates lines were used. (E) Quantitation of apoptotic/dead peripheral CD4⁺ T cells from the same CD4C/HIV-Nef^allele Tg mice whose analysis is shown in panel D. LN cells of Tg and non-Tg littermates were analyzed by FACS after staining with anti-CD4 monoclonal antibody and 7AAD. The data were pooled from results from Tg and non-Tg mice of each line as described for panel D and represent ratios (Tg/non-Tg) of the percentage of apoptotic/dead cells among the CD4⁺ T cells. Statistical analysis was performed with the Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.