TABLE 1.
Cell numbers in thymocyte subsets from Tg mice expressing different Nef alleles
| Mouse linea | Expression levelh | Absolute cell no. (106)b
|
Mean fluorescence (%)c of CD4 DP | |||
|---|---|---|---|---|---|---|
| Total | DP | SP CD4 | SP CD8 | |||
| Non-Tgd | 94 ± 21 | 80 ± 17 | 8.1 ± 1.5 | 3.9 ± 0.6 | 100 | |
| AD-93 | ||||||
| F115817 | L | 70 ± 27 | 60 ± 25 | 6.1 ± 1.4 | 1.2 ± 0.5e | 100 |
| F115820 | M | 16 ± 7e | 11 ± 6e | 4.1 ± 0.7e | 2.8 ± 0.9 | 60 ± 4e |
| 032an | ||||||
| F95581 | L | 30 ± 17e | 20 ± 12e | 3.4 ± 1.3e | 3.9 ± 3.3 | 58 ± 6e |
| F95582 | L-M | 53 ± 28f | 42 ± 21e | 5.4 ± 1.7 | 3.0 ± 0.9 | 63 ± 16f |
| 039nm | ||||||
| F75581 | M-H | 70 ± 27 | 62 ± 21 | 5.3 ± 1.9 | 1.3 ± 0.3e | 37 ± 4e |
| F51444 | M-H | 59 ± 19f | 44 ± 12e | 4.3 ± 1.8e | 2.5 ± 1.5 | 62 ± 12f |
| JR-CSF | ||||||
| F104995 | M-H | 48 ± 25e | 37 ± 20e | 2.6 ± 1.0e | 3.4 ± 3.0 | 45 ± 5e |
| F104996 | L-M | 100 ± 21 | 84 ± 19 | 4.2 ± 0.7e | 9.1 ± 3.0g | 25 ± 4e |
| YU10x | ||||||
| F92751 | H | 88 ± 18 | 75 ± 17 | 6.9 ± 1.0 | 2.1 ± 0.4 | 93 ± 21 |
| F92754 | L | 101 ± 12 | 84 ± 11 | 7.9 ± 3.3 | 3.3 ± 0.5 | 96 ± 13 |
| SF-2 | ||||||
| F92748 | L-M | 20 ± 10e | 12 ± 7e | 2.1 ± 1.0e | 2.1 ± 1.0 | 40 ± 16e |
| F92749 | L | 58 ± 19f | 47 ± 18e | 5.1 ± 1.4 | 2.1 ± 1.1 | 95 ± 4 |
| NL4-3(T71R) | ||||||
| F101376 | L | 65 ± 17f | 49 ± 13e | 8.1 ± 3.2 | 3.0 ± 1.0 | 78 ± 5f |
| F101374 | L | 66 ± 17f | 57 ± 13f | 4.9 ± 1.6e | 2.3 ± 2.3 | 69 ± 10f |
| F101371 | L | 81 ± 22 | 71 ± 20 | 4.8 ± 1.6e | 1.7 ± 0.7e | 93 ± 13 |
| F86707 | L-M | 67 ± 17f | 52 ± 13f | 3.0 ± 0.7e | 5.6 ± 1.9g | 44 ± 4e |
| Non-Tgd | 104 ± 26 | 90 ± 23 | 9.2 ± 3.1 | 2.4 ± 0.6 | 100 | |
| NL4-3(WT) | M-H | 69 ± 23e | 58 ± 20e | 2.9 ± 0.9e | 2.8 ± 0.6 | 36 ± 2e |
FACS analysis was performed on 4 to 10 mice (1.5 to 4 months old) for each Tg line, including the positive control CD4C/HIV-NefNL4-3(WT) Tg line. Data from Tg mice were always compared to those of their littermates done the same day. Older (4- to 6-month-old) Tg mice expressing Nef032an (F95582) or NefT71R (F101376) showed more severe depletion. For non-Tg mice, the total number of thymocytes is (53 ± 7.6) × 106 and the number of DP is (45 ± 5.9) × 106; for Nef032an Tg mice, the total number of thymocytes is (13.8 ± 10) × 106 and the number of DP is (11 ± 8.9) × 106; and for NefT71R Tg mice, the total number of thymocytes is (20.3 ± 9.5) × 106 and the number of DP is (17.4 ± 8.3) × 106 (six to eight mice in each group).
The absolute cell number was calculated by multiplying the total cell number in each organ by the percentage of each cell subset in this organ.
The mean fluorescences for CD4 were obtained by calculating the ratio of CD4 staining in Tg mouse thymuses relative to that of non-Tg mouse thymuses (100%) done in the same experiment, the same day. Mean values were then calculated with the values for each line.
The non-Tg control values were obtained by pooling the results of all non-Tg littermates from different lines.
Significant decrease (P < 0.01) according to Student's t test.
Significant decrease (P < 0.05) according to Student's t test.
Significant increase (P < 0.05) according to Student's t test.
L, low; M, medium; H, high; L-M, low to medium; M-H, medium to high.