FIG. 2.

Comparison of the abilities of T-ag and the KH512/513AA double mutant to oligomerize on the SV40 core origin. Band shift experiments, conducted with a ³²P-labeled 64-bp duplex oligonucleotide containing the SV40 core origin and either 3, 6, or 12 pmol of T-ag, are shown in lanes 2, 4, and 6, respectively. Reactions conducted with the same amounts of the KH512/513AA double mutant are shown in lanes 3, 5, and 7. The control reaction in lane 1 was conducted in the absence of protein. The positions of T-ag hexamers (H), double hexamers (DH), and free DNA (F) are indicated.