FIG. 1.

BTV entry inhibition by μ2 siRNA. HeLa cells were transfected with the μ2 siRNA for 48 h. Infection was performed as described in Materials and Methods. The effect of the siRNA was investigated by monitoring the cellular uptake of Tf-TRITC by confocal microscopy. BTV particles were stained with a polyclonal anti-VP5 primary antibody and localized with a FITC-conjugated secondary antibody. (A) HeLa cells transfected with the control siRNA, which does not inhibit either Tf uptake (a) or BTV entry (b). (B) Inhibitory effect of μ2 siRNA. In panel a, arrows indicate cells where Tf-TRITC uptake was inhibited, which show no punctate cytoplasmic staining. The arrow in panel c indicates a reduced number of internalized BTV particles in a cell affected by the siRNA.