FIG. 2.

Effect of siRNA on BTV replication. Transfection of HeLa cells with the μ2 siRNA was carried out for 36 h. Cells were infected with BTV, using an MOI of 0.5, and incubated for 12 h. Infected cells were harvested and counted, and the same number was loaded in each lane for Western blot analysis. (A) SDS-10% PAGE of infected cells stained with anti-VP5 and anti-μ2 antibodies. (B) Plaque assay analysis. Each sample was tested on BSR cells in triplicate as described in Materials and Methods. Results are presented as percentages, where the plaque number seen for untreated cells is taken as 100%.