FIG. 5.

Inhibition of endosomal acidification. The dependence of BTV entry on low pH was analyzed using increasing concentrations of BAF. Pretreated Vero cells were infected using an MOI of 1 and harvested after 24 h. (A) Cell lysates were analyzed by Western blotting using anti-VP5 and anti-β-tubulin antibodies. (B) Plaque assay of samples collected from BTV infection in the presence of BAF. (C) Confocal microscopy of infected Vero cells was performed as described in the legend to Fig. 3. Sample identification and final concentrations of BAF are indicated.