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. 2007 Feb 28;81(9):4664–4676. doi: 10.1128/JVI.01028-06

FIG. 3.

FIG. 3.

Rev R38 mutant env isoforms express different levels of Vpu but similar levels of Env irrespective of the Rev titer. (A) The R38 mutation introduces a premature translation termination codon corresponding to amino acid 38 in the second Rev exon of the env cDNA constructs. (B) The activity of Rev R38 env isoform mutants was measured by CAT assays 40 h after the cotransfection of HeLa cells with pCMVTat2X, pEGFP, and pDM128 with or without pLTRrev1. (C) Densitometry analysis of Env and Vpu expression from the env cDNA constructs. (D) Immunoblot assay of gp160 Env and Vpu synthesized in HeLa cells cotransfected with env cDNA, pCMVTat2X, pEGFP, pDM128, and increasing amounts of pLTRrev1. wt, wild-type sequence; R, R38 mutant sequence; Mk, mock (no env or pLTRrev1) transfection control; NL, HIVNL4.3 proviral plasmid control; E1R to E4R, env1 to env4 R38 mutant plasmids.