TABLE 2.
Primers and initial plasmid template for PCR mutagenesis
| Mutation | Initial PCR template | Primera
|
|||
|---|---|---|---|---|---|
| WT F | WT R | MUT F | MUT R | ||
| R38 | pNL4.3 | Odp.031 | Odp.081 | Odp.267 | Odp.268 |
| N20 | pDRenv2 | Odp.151 | Odp.080 | Odp.281 | Odp.282 |
| Rev− | pDRenv2 | Odp.002 | Odp.092 | Odp.263 | Odp.264 |
| Strengthening of Rev AUG codon | pDRenv2 | Odp.002 | Odp.092 | Odp.330 | Odp.331 |
| Addition of Kozak AUG codon | pDRenv5 or pDRenv6 | Odp.001 | Odp.092 | Odp.513 | Odp.514 |
| Change from Kozak AUG to AGG | pDRenv5 or pDRenv6 | Odp.001 | Odp.092 | Odp.515 | Odp.516 |
a
WT, wild-type; F, forward; R, reverse; MUT, mutagenic.