FIG. 1.

Ad association in blood. hCD46 transgenic mice were injected i.v. with 10¹¹ VP of an Ad5 vector. (A) Blood extracted 5 min after injection was immediately separated into blood cell and serum fractions. Total DNA was extracted from blood cell and serum fractions along with carrier DNA and analyzed for the presence of Ad genomes by quantitative PCR. (B) Platelets purified from blood 10 min after Ad delivery were subjected to TEM analysis. Virus particles associated with the external platelet membrane and membranes of the open canalicular system are shown (arrows). (C) Levels of soluble CD62p in sera were measured by ELISA 5 min and 6 h after Ad delivery. (D) Levels of D-dimer fibrinogen breakdown products in sera were measured by ELISA 5 min and 6 h after Ad delivery. Four mice per group were used.