FIG. 3.

Luciferase promoter assay for vTR (A) and chTR (B) in LMH and MSB-1 cells. Wild-type and mutant vTR/chTR promoter fragments were inserted upstream from the firefly luciferase reporter gene in the pGL3-Basic vector to generate pvTR and pchTR. Schematic diagrams of pvTR and pchTR are shown at the top. Black rectangles indicate Sp1 sites. An arrow indicates the transcription start sites of the vTR and chTR genes. Firefly luciferase activity was normalized with respect to the Renilla luciferase activity obtained from the cotransfected pRL plasmid. The luciferase activities of the pvTR and pchTR wild-type plasmids were normalized to 100%. The means and standard deviations of at least three independent experiments are shown. Values for the mutated promoter significantly different from those for the corresponding wild-type promoter are indicated by an asterisk.