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. 2007 Feb 21;81(9):4848–4857. doi: 10.1128/JVI.02530-06

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FIG. 5. — Functional study of Sp1 sites located close to the transcriptional start site in LMH and MSB-1 cells. (A) Comparison of pvTR and pvS-TR activities in LMH and MSB-1 cells. Schematic diagram of pvS-TR is shown. The promoter activity of the pvS-TR construct was assessed with the luciferase assay, normalized with respect to Renilla luciferase activity, and expressed relative to the activity of pvTR (the entire vTR promoter). The means and standard deviations of at least three independent experiments are shown. Values for pvS-TR significantly different from those for pvTR (indicated by an asterisk) and P values for nonparametric Kruskal-Wallis tests are shown. (B) Involvement of Sp1 sites in vTR transcriptional regulation. The promoter activity of pvS-TRΔSp1 was assessed with the luciferase assay, normalized with respect to Renilla luciferase activity, and expressed relative to the activity of pvS-TR (wild type). The means and standard deviations of at least three independent experiments are shown. Values for mutated promoters significantly different from those for the short vTR wild-type promoter are indicated by an asterisk.