TABLE 2.

Primers used to perform mutated TR promoters

Primer no.	type	Primer sequencesa
802	Forward	5′CTCGAGCCCTAACCCTAACCCCCCAAATTTTCACC3′
729	Reverse	5′AAGCTTGCCTTCCACCCGCCACGTGTG3′
M165	Forward	5′CTCGAGCAAGAGATCGGCGTTGCTTTC3′
M166	Reverse	5′AAGCTTGCCTTCCACCCGCCACGCGTG3′
782	Forward	5′CTCGAGTCCCCGCCGCCAATAGCTAC3′
730	Reverse	5′AAGCTTGCCTTCCACCCGCTTTTTTTGCCGGGGGAACCCCGCGTGGGGCTCTTG3′
718	Reverse	5′GGGGAACCCCGCGTGGGGCTCTTGTAGCTTTTTTCGCCTACGCCCACCGCGC3′
719	Reverse	5′AAGCTTGCCTTCCACCCGCCACGTGTGCCGGGGGAACCCCGCGTGGGGCTCTTGTA3′
700	Reverse	5′GAACCCCGCGTGGGGCTTTTTTTGCTTCCTCCGCCTAC3′
701	Reverse	5′AAGCTTGCCTTCCACCCGCCACGTGTGCCGGGGGAACCCCGCGTGGGGC3′
M270	Forward	5′CTCGAGTCTTTTTTGCCAATAGCTACGCGGCAGC3′
M271	Reverse	5′CCCCGCGTGGGGCTCTTGTAGCTTCCTCTTTTTTTTTTCACCGCGCGCCTATTG3′
M171	Reverse	5′GAACCCCGCGTGGGGCTTTTTTTGCCTCCTCCGCCTAC3′
M178	Reverse	5′AAGCTTGCCTTCCACCCGCCACGCGTGCCGGGGGAACCCCGCGTGGGGC3′
713	Forward	5′AAACTTGGATTATGCAAGTG3′
712	Reverse	5′CACTTGCATAATCCAAGTTTTTTTTACATCACAGGTGGTATGTG3′
715	Forward	5′GTACACCTGCCTGCACTACT3′
714	Reverse	5′AGTAGTGCAGGCAGGTGTACTTTTTCCTGTCGGCCGCGAGAGG3′
717	Forward	5′GCATGGGGCGTGGCGGGAGA3′
716	Reverse	5′TCTCCCGCCACGCCCCATGCTTTTTCCCCGCCCCTTCCTGTGG3′
703	Forward	5′AGCTACGCGGCAGCGTACAGCCCGG3′
702	Reverse	5′ACGCTGCCGCGTAGCTTTTTTCGGCGGGGAGGAGAGCG3′
705	Forward	5′AGGCGCGCGGTGGGCGTAGG3′
704	Reverse	5′TACGCCCACCGCGCGCCTTTTTTCCGGGCTGTACGCTGCC3′
M168	Forward	5′AGCGGGGCGGCAGCGTGCAGCCCGG3′
M167	Reverse	5′ACGCTGCCGCCCCGCTTTTTTCCGCGGGGAGGAGAGCGGG3′
M170	Forward	5′GGGCGCCCGGTGGGCGTAGG3′
M169	Reverse	5′TACGCCCACCGGGCGCCCTTTTTCCGGGCTGCACGCTGCC3′
804	Forward	5′TGGCGGGAGATGAATGACCG3′
M180	Forward	5′GCATCGGACCCCGCGGGCCCACAGGAAGGGG3′

^aPoint mutations with respect to the wild-type sequences of the vTR and chTR promoters are in bold and restriction sites are underlined.