TABLE 4.
Production of mutant constructs: distal mutations
| Constructsa | PCRb
|
||
|---|---|---|---|
| 1st amplification
|
2nd amplification
|
||
| Template | Primers | Primersb | |
| pvTRΔE3 | pvTR | 802/730 | |
| pvTRΔTATA | pvTR | 802/700 | 802/701 |
| pvS-TRΔSp1 | pvTR | M270/M271 | M270/719 |
| pvTRΔEBS | pvTR | 802/718 | 802/719 |
| pchTRΔTATA | pchTR | M165/M171 | M165/M178 |
| pvTRΔE2ΔE3 | pvTRΔE2 | 802/730 | |
| pvTRΔEBSΔE3 | pvTRΔEBS | 802/730 | |
a
The Δ symbol means that the following site specified is mutated. E2 and E3 correspond to E-box 2 and E-box 3, respectively.
b
Primer sequences are described in Table 2. Primers which comprise desired mutations are in bold.