Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
All five candidate domain 2 mutants fail to activate PKB. PKB activation by stably expressed candidate domain 2 mutants. An equivalent amount of extract (60 μg protein) from serum-starved CREF fibroblasts stably transfected with empty pBABE plasmid (vector) or pBABE plasmid coding for either wt E4-ORF1 (wt) or the indicated mutant E4-ORF1 were immunoblotted with antibodies to phospho-PKB (T308), PKB, or E4-ORF1.
