FIG. 1.

EBV reactivation induces DcR3 expression. (A) Latent EBV in P3HR1 cells was induced into the lytic cycle by TPA (40 ng/ml) and sodium butyrate (3 mM) treatment, and viral reactivation was monitored by the expression of Rta, EA-D, and Zta measured by Western analysis at 12-hour intervals in a 48-hour time course. Western blots were probed with antibodies to Rta, EA-D, Zta, or the control cellular protein β-actin. p.i., postinduction. (B) DcR3 in culture media was measured by ELISA at the indicated time points post-chemical induction. Averages and standard deviations were from measurements taken in triplicate.