TABLE 1.

Approaches to establish 22L scrapie infection in C2C12 myoblasts and myotubes

Method to establish infection in C2C12 cells	Outcome in:
	N2A cells	C2C12 cells
Add 22L scrapie brain homogenatea	Scrapie infection established	Scrapie infection not established
Coculture with 22L scrapie-infected N2A cells separated by a barrierb	Scrapie infection maintained	Scrapie infection not established
Coculture with 22L scrapie-infected N2A cells and Zeocin-transfected C2C12 cells	Death due to Zeocin sensitivity	Scrapie infection established in Zeocin-resistant myoblasts and myotubes

^aHomogenates at 0.1% to 1% (wt/vol).

^bOne- or 8-μm-pore-diameter membranes.