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. 2007 Feb 14;81(9):4625–4632. doi: 10.1128/JVI.02160-06

FIG. 2.

FIG. 2.

Heparin and HS inhibit HRV54 binding to RD cells. Cells were grown in 12-well plates, washed, and challenged with 35S-labeled HRV54 at ∼16,000 cpm in 0.4 ml HBSS with gentle rocking for 60 min at 34°C in the presence of glycans as indicated. Inhibition by heparin (H) was assessed at concentrations of between 0.25 and 2 mg/ml (A), and inhibition by the respective glycans was assessed at 2 mg/ml (B). Cells were washed to remove unbound virus, and cell-associated radioactivity was measured by liquid scintillation counting. Virus binding in the absence of the glycans (buffer) was set to 100%. Values are means from three independent experiments ± standard deviations.