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. 2007 Feb 14;81(9):4551–4563. doi: 10.1128/JVI.01366-06

FIG. 4.

FIG. 4.

Three EE markers, EEA1 (A and B), rabaptin 5 (C and D), and Rab4 (E and F), are associated with HCV NS4B. Full-length (C5B) replicon cells were grown for 48 h and processed for endogenous EEA1, rabaptin 5, or Rab4 fluorescence as described in Materials and Methods. EEA1, rabaptin 5, and Rab4 were stained with their respective antibodies and detected with Alexa Fluor 594-conjugated secondary antibody to EEA1, rabaptin 5, and Rab4. Notice the direct colocalization of NS4B with EEA1 (B), rabaptin-5 (D), or Rab4 (F), as well as the redistribution of these markers in cells with the replicon. Bars = 10 μm.