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. 2007 Feb 14;81(9):4473–4481. doi: 10.1128/JVI.02498-06

FIG. 1.

FIG. 1.

In vitro growth of rotaviruses encoding wt and defective forms of NSP1. (A) Representation of the NSP1 forms made by different rotavirus isolates (strain name in parentheses), including the location of putative RING and IRF3-binding domains, and cytoskeleton localization signal. The variants SA11-5S, SA11-30-1A, and brvA encode C-truncated forms of NSP1 and are sister strains of the wt viruses SA11-4F (SA11g4“O”), SA11-30-19, and bovine UK (26, 32a). GenBank accession numbers for the gene 5 sequences: AAK14071 (SA11-4F), AAK14072 (SA11-5S), AAK14069 (SA11-30-19), AAK14070 (SA11-30-1A), AAA18011 (UK), and AAA18012 (brvA). (B) Virus titers produced by infecting the indicated cell lines at an MOI of 3 with rotavirus strains encoding wt and defective NSP1. Cells were harvest at times of maximum virus yield (24 h p.i. for MA104 cells and 48 h p.i. for Caco-2 and FRhL2 cells). Titers were determined by plaque assay on MA104 cells and represent the averaged values obtained for replicate parallel infections.