FIG. 5.
Heterologous prime-boost studies with mice. Naïve mice (n = 4/group) were primed on day 0 with (A) 109 vp rAd35-Gag, (B) 109 vp rAd26-Gag, or (C) 50 μg DNA-Gag and then boosted on day 28 with 109 vp of the rAd-Gag vectors shown. Arrows indicate immunizations. Gag-specific cellular immune responses were assessed by Db/AL11 tetramer binding assays at multiple time points following immunization. (D, E) Serum Ad5-, Ad11-, Ad35-, Ad50-, and Ad26-specific NAb titers were determined using the mice in the study whose results are depicted in panel (A). (F, G) Serum Ad5-, Ad35-, Ad26-, Ad48-, and Ad49-specific NAb titers were determined using the mice in the study whose results are depicted in panel (B). Mean responses with standard errors are shown.