FIG. 3.

In vivo [³²P]orthophosphate radiolabeling of emerin isoforms. Mock-infected or HSV-1-infected cells were radiolabeled with [³²P]orthophosphate beginning at 1 h p.i and harvested at 16 h p.i. Samples were prepared as described in Materials and Methods. Total samples from mock-infected and infected lysates (a) or immunoprecipitates from soluble extracts using anti-emerin antibody (b) were then analyzed by SDS-PAGE and autoradiography. The upper panels are autoradiographs, whereas the lower panels are Western blots from identical gels with probed with anti-emerin antibody. The results confirm the presence of the U₀/U₁ isoforms and the novel I₁ and I₂ forms as phosphorylated species.