FIG. 4.

Examination of the effect of inhibitors on HSV-induced emerin phosphorylation. Replicate cultures of HSV-1-infected cells were supplemented with different kinase inhibitors at various concentrations at 4 h p.i. Cells were harvested 8 h p.i., lysed, and analyzed by Western blotting with anti-emerin antibody. The inhibitors shown here were STS and the PKC inhibitor Ro31-7549 (Ro-31), with concentrations indicated in nM, and the cdc2 inhibitor olomoucine (Olo) concentrations, with concentrations indicated in μM.